LC3 Polyclonal antibody

LC3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12135-1-AP

Synonyms



Tested Applications

Positive WB detected inChloroquine treated HEK-293 cells, Chloroquine treated HeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inStarvation treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12135-1-AP targets LC3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, pig, turtle
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag2781
Full Name microtubule-associated protein 1 light chain 3 alpha
Calculated molecular weight 121 aa, 14 kDa
GenBank accession numberBC015810
Gene symbol MAP1LC3A
Gene ID (NCBI) 84557
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 kDa).

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 12135-1-APDownload protocol
IHC protocol for LC3 antibody 12135-1-APDownload protocol
IF protocol for LC3 antibody 12135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Autophagy is essential for mouse sense of balance.

Authors - Mari?o Guillermo G
mouseWB

Autophagy

Assessing autophagy in the context of photodynamic therapy.

Authors - Reiners John J JJ Jr
mouseWB

Cell Death Differ

ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.

Authors - Isaac Tamargo-Gómez
humanWB

EMBO J

Unsaturated fatty acids induce non-canonical autophagy.

Authors - Mireia Niso-Santano
humanWB

Cell Mol Gastroenterol Hepatol

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Authors - Linqiang Zhang
mouseWB

Chem Biol

Thiopeptide Antibiotics Exhibit a Dual Mode of Action against Intracellular Pathogens by Affecting Both Host and Microbe.

Authors - Qingfei Zheng