LC3 Antibody 27 Publications

Rabbit Polyclonal| Catalog number: 12135-1-AP

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Freight/Packing

Con: 30 μg/150 μl

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Species specificity:
human, mouse, rat, zebrafish, pig

Positive WB detected in:
Chloroquine treated HeLa cells, Chloroquine treated HEK-293 cells, human brain tissue, mouse brain tissue, rat brain tissue

Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue

Positive IF detected in:
Starvation treated HEK-293 cells

Recommended dilution:
WB : 1:500-1:800
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
microtubule-associated protein 1 light chain 3 alpha

Calculated molecular weight:
121aa,14 kDa

Observed molecular weight:
16 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MAP1LC3A

Synonyms
LC3, LC3A, LC3A/B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A
Background

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 kDa).


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