LC3A-Specific Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 18722-1-AP

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 61 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, human brain tissue, mouse testis tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:4000
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
microtubule-associated protein 1 light chain 3 alpha

Calculated molecular weight:
14 kDa

Observed molecular weight:
15-18kd

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MAP1LC3A

Synonyms
LC3, LC3A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A
Background

LC3A, also named as MAP1LC3A, LC3, MAP1ALC3 and MAP1BLC3, belongs to the MAP1 LC3 family. LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B which are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. (PMID:11060023) MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.(PMID:12740394 ) This antibody is specific to LC3A.It recognize both LC3A-I and LC3A-II.


Back
to top