Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human prostate cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15695-1-AP targets MAP1S in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAP1S fusion protein Ag8315|
|Full Name||microtubule-associated protein 1S|
|Calculated molecular weight||806 aa, 85 kDa|
|Observed molecular weight||130-150 kDa|
|GenBank accession number||BC008806|
|Gene ID (NCBI)||55201|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S.
|Product Specific Protocols|
|WB protocol for MAP1S antibody 15695-1-AP||Download protocol|
|IHC protocol for MAP1S antibody 15695-1-AP||Download protocol|
|IF protocol for MAP1S antibody 15695-1-AP||Download protocol|
|IP protocol for MAP1S antibody 15695-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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