MEK2 Monoclonal antibody
MEK2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, Mouse, Rat and More (2)
WB, IHC, IF, ELISA
Cat no : 67410-1-Ig
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1, Neuro-2a cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67410-1-Ig targets MEK2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MEK2 fusion protein Ag29871|
|Full Name||mitogen-activated protein kinase kinase 2|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||44-47 kDa|
|GenBank accession number||BC018645|
|Gene ID (NCBI)||5605|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
|Product Specific Protocols|
|WB protocol for MEK2 antibody 67410-1-Ig||Download protocol|
|IHC protocol for MEK2 antibody 67410-1-Ig||Download protocol|
|IF protocol for MEK2 antibody 67410-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Mol Cell Biochem
Anti-inflammatory effects of extracellular vesicles from Morchella on LPS-stimulated RAW264.7 cells via the ROS-mediated p38 MAPK signaling pathway.
A potent tumor-selective ERK pathway inactivator with high therapeutic index.
Signal Transduct Target Ther
IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas.
Proc Natl Acad Sci U S A
ERK and c-Myc signaling in host-derived tumor endothelial cells is essential for solid tumor growth