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TAK1 Antibody

TAK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12330-2-AP

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a



Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells, CHO cells, SKOV-3 cells, K-562 cells, A375 cells, A431 cells, COLO 320 cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

12330-2-AP targets TAK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, hamster, mouse samples.

Tested Reactivity human, hamster, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAK1 fusion protein Ag3041
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579aa,64 kDa
Observed molecular weight 64-67 kDa, 75-80 kDa
GenBank accession number BC017715
Gene symbol MAP3K7
Gene ID (NCBI) 6885
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7 – mitogen-activated 

protein kinase kinase kinase 7 – is a serine/threonine protein kinase triggering phosphorylation of AMP-activated

 protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic proteins 

(BMPs), and various proinflammatory mediators.


What is the molecular weight of TAK1? Are there any isoforms of TAK1?

The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents 

the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their 

expression levels across tissues (PMID: 11118615).


Is TAK1 post-translationally modified?

TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding to TABs triggers 

autophosphorylation of TAK1 and depends on polyubiquitination of receptor-interacting protein 1 (RIP1) 

(PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated, 

which impacts its activity (PMID: 28106845).


What is the subcellular localization of TAK1?

TAK1 is present in the cytoplasm.


What is the tissue expression pattern of TAK1?

TAK1 is ubiquitously expressed.


How can TAK1 activity be monitored?

TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on 

Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187 

phosphorylation.


Protocols

Product Specific Protocols
WB protocol for TAK1 antibody 12330-2-APDownload protocol
IHC protocol for TAK1 antibody 12330-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Mol Cell Biol

TRIM14 promotes endothelial activation via activating NF-κB signaling pathway.

Authors - Xuan Huang
humanWB

Onco Targets Ther

JAZF1 Suppresses Papillary Thyroid Carcinoma Cell Proliferation and Facilitates Apoptosis via Regulating TAK1/NF-κB Pathways.

Authors - Liangliang Huang
humanWB

J Cancer

Recombinant Dual-target MDM2/MDMX Inhibitor Reverses Doxorubicin Resistance through Activation of the TAB1/TAK1/p38 MAPK Pathway in Wild-type p53 Multidrug-resistant Breast Cancer Cells.

Authors - Yangwei Fan
humanWB

Cancer Biol Ther

Dual-target MDM2/MDMX inhibitor increases the sensitization of doxorubicin and inhibits migration and invasion abilities of triple-negative breast cancer cells through activation of TAB1/TAK1/p38 MAPK pathway.

Authors - Yangwei Fan
humanWB

Amino Acids

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Authors - Chen Bai
humanWB

J Immunol

NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.

Authors - Zhangchuan Xia