MAP3K7 Monoclonal antibody

MAP3K7 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A5B2

Cat no : 67707-1-Ig

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a



Tested Applications

Positive WB detected inA431 cells, NIH/3T3 cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67707-1-Ig targets MAP3K7 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP3K7 fusion protein Ag21286
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579 aa, 64 kDa
Observed molecular weight67-70 kDa
GenBank accession numberBC017715
Gene symbol MAP3K7
Gene ID (NCBI) 6885
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP3K7 antibody 67707-1-IgDownload protocol
IHC protocol for MAP3K7 antibody 67707-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols