Validation Data Gallery
|Positive WB detected in
|A431 cells, NIH/3T3 cells, HepG2 cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells
|Positive IHC detected in
|human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IHC : 1:250-1:1000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
67707-1-Ig targets TAK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Human, Mouse, Rat
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|TAK1 fusion protein Ag21286
|mitogen-activated protein kinase kinase kinase 7
|Calculated molecular weight
|579 aa, 64 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.
Cell Death Dis
USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1
Liensinine reduces acute lung injury brought on by lipopolysaccharide by inhibiting the activation of the NF-κB signaling pathway through modification of the Src/TRAF6/TAK1 axis
Biochim Biophys Acta Mol Cell Res
IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5
Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.
TRIM44 aggravates cardiac fibrosis after myocardial infarction via TAK1 stabilization