TAK1 Monoclonal antibody

TAK1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A5B2

Cat no : 67707-1-Ig

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

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Validation Data Gallery

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  WB analysis using 67707-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67707-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67707-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67707-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

67707-1-Ig targets TAK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TAK1 fusion protein Ag21286
• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated molecular weight: 579 aa, 64 kDa
• Observed molecular weight: 67-70 kDa
• GenBank accession number: BC017715
• Gene symbol: MAP3K7
• Gene ID (NCBI): 6885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

Publications

Species	Application	Title
rat	IF	Neurochem Res; Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.; Authors - Xuehua Sun