JNK1/2/3 Polyclonal antibody

JNK1/2/3 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IP, IF

Conjugate

Unconjugated

Cat no : 51153-1-AP

Synonyms

c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK



Tested Applications

Positive WB detected inHeLa cells, A549 cells, COS-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 51153-1-AP is JNK1/2/3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK1/2/3 fusion protein Ag1442
Full Name mitogen-activated protein kinase 9
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC032539
Gene symbol MAPK9
Gene ID (NCBI) 5601
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Protocols

Product Specific Protocols
WB protocol for JNK1/2/3 antibody 51153-1-APDownload protocol
IF protocol for JNK1/2/3 antibody 51153-1-APDownload protocol
IP protocol for JNK1/2/3 antibody 51153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rabbitWB

Microb Pathog

Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis.

Authors - Liyan Chen
humanWB

Biomed Pharmacother

Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.

Authors - Ya-Sai Sun
humanWB

Nat Commun

SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.

Authors - Peiyuan Zhang