Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, COS-7 cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells, HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
51153-1-AP targets JNK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||JNK fusion protein Ag1442|
|Full Name||mitogen-activated protein kinase 9|
|Calculated molecular weight||424 aa, 48 kDa|
|Observed molecular weight||44-55 kDa|
|GenBank accession number||BC032539|
|Gene ID (NCBI)||5601|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.
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