JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 51153-1-AP

Synonyms

MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24

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Validation Data Gallery

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  WB analysis of HeLa using 51153-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 51153-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 51153-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 51153-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 51153-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51153-1-AP

  IP result of anti-JNK (IP:51153-1-AP, 4ug; Detection:51153-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 51153-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51153-1-AP (JNK antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 51153-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51153-1-AP (JNK antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 51153-1-AP

  Immunofluorescent analysis of HeLa cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HUVEC using 51153-1-AP

  Immunofluorescent analysis of HUVEC cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, COS-7 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

51153-1-AP targets JNK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag1442
• Full Name: mitogen-activated protein kinase 9
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC032539
• Gene Symbol: JNK
• Gene ID (NCBI): 5601
• RRID: AB_10898168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publications

Species	Application	Title
human	WB	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
rat	WB	Neurochem Res; Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.; Authors - Xuehua Sun
rabbit	WB	Microb Pathog; Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis.; Authors - Liyan Chen