TAU Antibody 10274-1-AP

Tested Applications

Applications:

ELISA, IF, IHC, WB
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SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 10274-1-AP (TAU antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 40x lens)

Species specificity:

human, mouse, rat

Positive WB detected in:

SH-SY5Y cells, HeLa cells, HT-1080 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells

Positive IHC detected in:

human brain tissue, human brain tissue, human gliomas tissue, human lung cancer tissue

Positive IF detected in:

mouse brain tissue

Recommended dilution:

WB : 1:500-1:2000

IHC : 1:20-1:200

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

TAU fusion protein Ag0354

Full name:

microtubule-associated protein tau

Calculated molecular weight:

79 kDa

Observed molecular weight:

68 kDa, 81 kDa,100 kDa

GenBank accession number:

BC000558

Gene ID (NCBI):

4137

Gene symbol

MAPT

Synonyms

DDPAC, FLJ31424, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

Background

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Present polyclonal anti-MAPT antibody was produced by immunizing animals with N-terminal of MAPT and can detect approx 70-kDa and 100-kDa bands in brain tissues.

Showing 1- [3] of [3] Publications

Author:		Rodríguez-Navarro Jose A JA
Pubmed ID:		18640988
Journal:		Hum Mol Genet
Title:		Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice.
Application:		IHC

Species:		mouse

Author:		Yang Ji Yeon JY
Pubmed ID:		20649952
Journal:		BMC Cancer
Title:		p-Glycoprotein ABCB5 and YB-1 expression plays a role in increased heterogeneity of breast cancer cells: correlations with cell fusion and doxorubicin resistance.
Application:		WB

Species:		human

Author:		Feixiang Wang
Pubmed ID:		28328017
Journal:		Cell Biol Int
Title:		Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease.
Application:		WB

Species:		human

WB Figures

HeLa cells were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HT-1080 cells were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10274-1-AP(TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 40x lens)

Immunohistochemical of paraffin-embedded human gliomas using 10274-1-AP(TAU antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human gliomas using 10274-1-AP(TAU antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human lung cancer using 10274-1-AP(TAU antibody) at dilution of 1:100 (under 25x lens)

IF Figures

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 10274-1-AP (TAU antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TAU Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 10274-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [3] of [3] Publications

WB Figures

IHC Figures

IF Figures

TAU Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 10274-1-AP

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Related Primary Antibodies

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Tested Applications

Product Information

Immunogen Information

Background