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TAU Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10274-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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TAU Antibody WB SH-SY5Y cells 10274-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

TAU Antibody IF mouse brain tissue 10274-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 10274-1-AP (TAU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TAU Antibody IHC human brain tissue 10274-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 10x lens)

TAU Antibody IHC human brain tissue 10274-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP( TAU Antibody) at dilution of 1:200 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
SH-SY5Y cells, HeLa cells, HT-1080 cells, rat brain tissue
Positive IHC detected in:
human brain tissue, human gliomas tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue,
Recommended dilution:
WB : 1:300-1:1500
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TAU fusion protein Ag0354
Full name:
microtubule-associated protein tau
Calculated molecular weight:
37-46, 79-81 kDa
Observed molecular weight:
66-70 kDa, 100 kDa
GenBank accession number:
BC000558
Gene ID (NCBI):
4137
Gene symbol
MAPT
Synonyms
DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

Background

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of TAU exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-MAPT antibody was produced by immunizing animals with N-terminal of MAPT and can detect approx 70-kDa and 100-kDa bands in brain tissues.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TAU Rabbit Polyclonal antibody

Tanusree (Verified Customer), 02-14-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:100
No image submitted with review

No image submitted with review

Cell/Tissue Type
Mouse brain
Lot Number
The antibody worked well with the mouse primary cells and tissue.

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