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MAVS; VISA Polyclonal antibody
MAVS; VISA Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14341-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, Jurkat cells, HeLa cells, HuH-7 cells, HepG2 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14341-1-AP targets MAVS; VISA in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAVS; VISA fusion protein Ag5655|
|Full Name||mitochondrial antiviral signaling protein|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||50-55 kDa, 70-75 kDa|
|GenBank accession number||BC044952|
|Gene ID (NCBI)||57506|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
|Product Specific Protocols|
|WB protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IHC protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IF protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IP protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.
An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation
MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.
J Exp Med
TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination.
CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Damien (Verified Customer) (09-03-2019)
No convince by this anti-MAVS antibody.In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.