MBP-Tag Polyclonal antibody

MBP-Tag Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15089-1-AP

Synonyms

Maltose Binding Protein, MBP, MBP Tag


"MBP-Tag Antibodies" Comparison

View side-by-side comparison of MBP-Tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • Western Blot (WB) analysis of Recombinant protein using MBP-Tag Polyclonal antibody (15089-1-AP)

    WB analysis of Recombinant protein using 15089-1-AP

    lane1: MBP-NOL6 64kd; lane2 MBP 42kd were subjected to SDS PAGE followed by western blot with 15089-1-AP (MBP-Tag Antibody) at dilution of 1:3000.


Tested Applications

Positive WB detected inlane1: MBP-NOL6 64kd; lane2 MBP 42kd, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

15089-1-AP targets MBP-Tag in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBP-Tag fusion protein Ag0942
Full Name MBP-Tag
Calculated molecular weight 40 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Publications

SpeciesApplicationTitle

Nature

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Authors - Zhaoyang Li
ELISA

Blood

Gamma-glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins.

Authors - Zhenyu Hao
WB

Blood

γ-Glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins.

Authors - Zhenyu Hao
WB

Sci Adv

The deubiquitinases UBP12 and UBP13 integrate with the E3 ubiquitin ligase XBAT35.2 to modulate VPS23A stability in ABA signaling.

Authors - Guangchao Liu
WB

Cell Res

Modulation of microRNA processing by mismatch repair protein MutLα.

Authors - Mao Guogen G

Proc Natl Acad Sci U S A

Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology.

Authors - Jianguo Yang