MCOLN3 Polyclonal antibody

• 

  WB analysis using 13879-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13879-1-AP (Mucolipin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MCOLN3 Polyclonal Antibody for WB, ELISA

Cat No. 13879-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Synonyms

Mucolipin 3, Transient receptor potential channel mucolipin 3, TRP ML3, TRPML3

---

Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

13879-1-AP targets MCOLN3 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCOLN3 fusion protein Ag4962
• Full Name: mucolipin 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC060765
• Gene Symbol: MCOLN3
• Gene ID (NCBI): 55283
• RRID: AB_2142973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TDD5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCOLN3 (Mucolipin 3) is a member of the TRPML (Transient Receptor Potential Mucolipin) gene family and encodes a non-selective cation channel protein that is primarily localized on the membranes of endosomes and lysosomes. MCOLN3 promotes endosome acidification, regulates membrane transport and fusion, and is involved in the autophagy process. Additionally, MCOLN3 is involved in the regulation of intracellular calcium (Ca²⁺) and iron (Fe²⁺) ion homeostasis, maintains lysosomal membrane potential, promotes endosome-lysosome fusion and material transport (such as autophagy), and may also be involved in neuronal signaling and immune regulation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Traffic; The Ca(2+) channel TRPML3 regulates membrane trafficking and autophagy.; Authors - Hyun Jin Kim ; KD Validated
mouse,human	WB,IF,CoIP	Cell Mol Gastroenterol Hepatol; FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis; Authors - Xitang Li
human	WB	Phytomedicine; Baicalin induces cell death of non-small cell lung cancer cells via MCOLN3-mediated lysosomal dysfunction and autophagy blockage; Authors - Xian Dong ; KD Validated