• Featured Product
  • KD/KO Validated

MDMX Polyclonal antibody

MDMX Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17914-1-AP

Synonyms

Double minute 4 protein, HDMX, Mdm2 like p53 binding protein, MDM4, MDMX, MRP1, p53 binding protein Mdm4, Protein Mdm4, Protein Mdmx



Tested Applications

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

17914-1-AP targets MDMX in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDMX fusion protein Ag12319
Full Name Mdm4 p53 binding protein homolog (mouse)
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC067299
Gene symbol MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MDMX antibody 17914-1-APDownload protocol
IP protocol for MDMX antibody 17914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Breast Cancer Res

Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells.

Authors - Chong Gao
  • KD Validated
humanWB

Am J Cancer Res

MiR-1205 functions as a tumor suppressor by disconnecting the synergy between KRAS and MDM4/E2F1 in non-small cell lung cancer.

Authors - Hong Yan
  • KD Validated
humanWB

Front Oncol

Long Non-coding RNA SNHG12 Functions as a Competing Endogenous RNA to Regulate MDM4 Expression by Sponging miR-129-5p in Clear Cell Renal Cell Carcinoma.

Authors - Zhipeng Wu
humanWB

Oncoscience

PMID: 26973857

Authors - Matthew Smonskey
humanWB

Oncotarget

PMID: 28052008

Authors - Chaojun Xue
humanWB

Genet Mol Res

PMID: 27706681

Authors - Y Q Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Breanna (Verified Customer) (02-18-2020)

It works well

  • Applications: ChIP,
FH

Breanna (Verified Customer) (01-29-2020)

Works well

  • Applications: Immunoprecipitation,Flow Cytometry,