MDMX/MDM4 Polyclonal antibody

• 

  WB analysis of HeLa using 17914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 17914-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 17914-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 17914-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17914-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17914-1-AP

  IP result of anti-MDMX (IP:17914-1-AP, 4ug; Detection:17914-1-AP 1:600) with HeLa cells lysate 1120 ug.

• 

  IHC staining of human ovary tumor using 17914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 17914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17914-1-AP

Publications(35)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MDM4, MDMX, HDMX, Mdm2-like p53-binding protein, p53-binding protein Mdm4

---

Tested Applications

• Positive WB detected in: HeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17914-1-AP targets MDMX/MDM4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDMX/MDM4 fusion protein Ag12319
• Full Name: Mdm4 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 490 aa, 55 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC067299
• Gene Symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• RRID: AB_2143683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IF	Brain; Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.; Authors - Hyojung Kim
human	WB	Cancer Res; FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.; Authors - Xiang Ling
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang
human	WB	Cell Death Discov; LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX; Authors - Qingqing Zhao
human	WB	J Med Chem; Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.; Authors - Shiyan Zhang
mouse	WB	PLoS Genet; MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.; Authors - Meenalakshmi Chinnam ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Breanna (Verified Customer) (02-18-2020)

It works well

• Applications: ChIP,

FH

Breanna (Verified Customer) (01-29-2020)

Works well

• Applications: Immunoprecipitation, Flow Cytometry,