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MDMX Polyclonal antibody

MDMX Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17914-1-AP

Synonyms

Double minute 4 protein, HDMX, Mdm2 like p53 binding protein, MDM4, MDMX, MRP1, p53 binding protein Mdm4, Protein Mdm4, Protein Mdmx



Tested Applications

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

17914-1-AP targets MDMX in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDMX fusion protein Ag12319
Full Name Mdm4 p53 binding protein homolog (mouse)
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC067299
Gene symbol MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MDMX antibody 17914-1-APDownload protocol
IP protocol for MDMX antibody 17914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Brain

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Authors - Hyojung Kim
humanWB

Cancer Res

FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.

Authors - Xiang Ling
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
humanWB

J Med Chem

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.

Authors - Shiyan Zhang
humanIP

Cell Death Dis

Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells.

Authors - W Wu
humanWB

Bioorg Chem

Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.

Authors - Dongjuan Si

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Breanna (Verified Customer) (02-18-2020)

It works well

  • Applications: ChIP,
FH

Breanna (Verified Customer) (01-29-2020)

Works well

  • Applications: Immunoprecipitation, Flow Cytometry,