|Positive WB detected in||HeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
17914-1-AP targets MDMX in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MDMX fusion protein Ag12319|
|Full Name||Mdm4 p53 binding protein homolog (mouse)|
|Calculated molecular weight||490 aa, 55 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC067299|
|Gene ID (NCBI)||4194|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.
Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.
FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.
Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.
J Med Chem
Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.
Cell Death Dis
Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells.
Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Breanna (Verified Customer) (02-18-2020)
It works well
Breanna (Verified Customer) (01-29-2020)