rat brain tissue were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 29900-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of mouse colon using 29900-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29900-1-AP (METT5D1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunohistochemistry (IHC)
IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
29900-1-AP targets METT5D1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
rat brain tissue were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 29900-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse colon using 29900-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29900-1-AP (METT5D1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 29900-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29900-1-AP (METT5D1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
METT5D1 Polyclonal antibody
Catalog Number
29900-1-AP
Citations
-
Dilutions
WB : 1:1000-1:8000 IHC : 1:400-1:1600
Applications
WB, IHC, ELISA
Reactivity
human, rat, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
