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METTL1 Polyclonal antibody
METTL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14994-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HuH-7 cells |
Positive IP detected in | A2780 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 10 publications below |
WB | See 8 publications below |
IHC | See 5 publications below |
Product Information
14994-1-AP targets METTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | METTL1 fusion protein Ag6980 |
Full Name | methyltransferase like 1 |
Calculated molecular weight | 31 kDa |
Observed molecular weight | 31 kDa |
GenBank accession number | BC000550 |
Gene symbol | METTL1 |
Gene ID (NCBI) | 4234 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.
Protocols
Product Specific Protocols | |
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WB protocol for METTL1 antibody 14994-1-AP | Download protocol |
IHC protocol for METTL1 antibody 14994-1-AP | Download protocol |
IF protocol for METTL1 antibody 14994-1-AP | Download protocol |
IP protocol for METTL1 antibody 14994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Lett Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.
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Front Immunol A Comprehensive Analysis of METTL1 to Immunity and Stemness in Pan-Cancer.
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Cancer Commun (Lond) Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression.
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PLoS Genet tRNA Modifying Enzymes, NSUN2 and METTL1, Determine Sensitivity to 5-Fluorouracil in HeLa Cells.
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Mol Ther METTL1/WDR4 mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hannah (Verified Customer) (12-05-2018) | Single specific band detected at expected size after overnight primary incubation.
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