|Positive WB detected in||A549 cells, HuH-7 cells|
|Positive IP detected in||A2780 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14994-1-AP targets METTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||METTL1 fusion protein Ag6980|
|Full Name||methyltransferase like 1|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC000550|
|Gene ID (NCBI)||4234|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.
|Product Specific Protocols|
|WB protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IHC protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IF protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IP protocol for METTL1 antibody 14994-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.
Cancer Cell Int
Prognostic role of METTL1 in glioma.
A Comprehensive Analysis of METTL1 to Immunity and Stemness in Pan-Cancer.
Cancer Commun (Lond)
Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression.
tRNA Modifying Enzymes, NSUN2 and METTL1, Determine Sensitivity to 5-Fluorouracil in HeLa Cells.
METTL1/WDR4 mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (12-05-2018)
Single specific band detected at expected size after overnight primary incubation.