• Featured Product
  • KD/KO Validated

METTL1 Polyclonal antibody

METTL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14994-1-AP

Synonyms

C12orf1, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w



Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, HeLa cells, rat brain tissue, mouse brain tissue
Positive IP detected inA2780 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14994-1-AP targets METTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL1 fusion protein Ag6980
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000550
Gene symbol METTL1
Gene ID (NCBI) 4234
RRIDAB_2142013
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

Protocols

Product Specific Protocols
WB protocol for METTL1 antibody 14994-1-APDownload protocol
IHC protocol for METTL1 antibody 14994-1-APDownload protocol
IF protocol for METTL1 antibody 14994-1-APDownload protocol
IP protocol for METTL1 antibody 14994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Structural basis of regulated m7G tRNA modification by METTL1-WDR4

Authors - Jiazhi Li
  • KO Validated
humanWB

Cell Res

Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.

Authors - Lionel Malbec
human,mouseWB,IHC

Mol Cell

N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Authors - Zihao Dai
  • KD Validated
human,mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
  • KD Validated
mouse

Mol Cell

Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Authors - Shuibin Lin
  • KO Validated
human,mouseIHC

Nat Commun

N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.

Authors - Hui Han
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhongwen (Verified Customer) (09-25-2023)

Clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
METTL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14994-1-AP)
FH

Hannah (Verified Customer) (12-05-2018)

Single specific band detected at expected size after overnight primary incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human fibroblast
METTL1 Antibody Western Blot, validation (1/1000 dilution) in human fibroblast (Cat no:14994-1-AP)