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  • KD/KO Validated

METTL1 Polyclonal antibody

METTL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14994-1-AP


C12orf1, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, HeLa cells, rat brain tissue, mouse brain tissue
Positive IP detected inA2780 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14994-1-AP targets METTL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL1 fusion protein Ag6980
Full Name methyltransferase like 1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC000550
Gene Symbol METTL1
Gene ID (NCBI) 4234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.


Product Specific Protocols
WB protocol for METTL1 antibody 14994-1-APDownload protocol
IHC protocol for METTL1 antibody 14994-1-APDownload protocol
IF protocol for METTL1 antibody 14994-1-APDownload protocol
IP protocol for METTL1 antibody 14994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Structural basis of regulated m7G tRNA modification by METTL1-WDR4

Authors - Jiazhi Li
  • KO Validated

Cell Res

Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.

Authors - Lionel Malbec

Mol Cell

N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Authors - Zihao Dai
  • KD Validated

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
  • KD Validated

Mol Cell

Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Authors - Shuibin Lin
  • KO Validated

Nat Commun

N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.

Authors - Hui Han
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Zhongwen (Verified Customer) (09-25-2023)

Clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
METTL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14994-1-AP)

Hannah (Verified Customer) (12-05-2018)

Single specific band detected at expected size after overnight primary incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human fibroblast
METTL1 Antibody Western Blot, validation (1/1000 dilution) in human fibroblast (Cat no:14994-1-AP)