• Featured Product
  • KD/KO Validated

METTL1 Polyclonal antibody

METTL1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14994-1-AP

Synonyms

C12orf1, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w



Tested Applications

Positive WB detected inA549 cells, HuH-7 cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14994-1-AP targets METTL1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL1 fusion protein Ag6980
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000550
Gene symbol METTL1
Gene ID (NCBI) 4234
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

Protocols

Product Specific Protocols
WB protocol for METTL1 antibody 14994-1-APDownload protocol
IHC protocol for METTL1 antibody 14994-1-APDownload protocol
IF protocol for METTL1 antibody 14994-1-APDownload protocol
IP protocol for METTL1 antibody 14994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.

Authors - Chen Wang
  • KD Validated
humanWB

PLoS Genet

tRNA Modifying Enzymes, NSUN2 and METTL1, Determine Sensitivity to 5-Fluorouracil in HeLa Cells.

Authors - Mayumi Okamoto
  • KD Validated
mouse

Mol Cell

Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Authors - Shuibin Lin
  • KO Validated
humanWB

Cell Res

Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.

Authors - Lionel Malbec

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hannah (Verified Customer) (12-05-2018)

Single specific band detected at expected size after overnight primary incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human fibroblast
METTL1 Antibody Western Blot, validation (1/1000 dilution) in human fibroblast (Cat no:14994-1-AP)