|Positive WB detected in||A549 cells, HuH-7 cells|
|Positive IP detected in||A2780 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14994-1-AP targets METTL1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||METTL1 fusion protein Ag6980|
|Full Name||methyltransferase like 1|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC000550|
|Gene ID (NCBI)||4234|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.
|Product Specific Protocols|
|WB protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IHC protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IF protocol for METTL1 antibody 14994-1-AP||Download protocol|
|IP protocol for METTL1 antibody 14994-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.
tRNA Modifying Enzymes, NSUN2 and METTL1, Determine Sensitivity to 5-Fluorouracil in HeLa Cells.
Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.
Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (12-05-2018)
Single specific band detected at expected size after overnight primary incubation.