|Positive WB detected in
|A549 cells, HuH-7 cells, HeLa cells, rat brain tissue, mouse brain tissue
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1000-1:4000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14994-1-AP targets METTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|METTL1 fusion protein Ag6980
|methyltransferase like 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.
|Product Specific Protocols
|WB protocol for METTL1 antibody 14994-1-AP
|IHC protocol for METTL1 antibody 14994-1-AP
|IF protocol for METTL1 antibody 14994-1-AP
|IP protocol for METTL1 antibody 14994-1-AP
|Click here to view our Standard Protocols
Structural basis of regulated m7G tRNA modification by METTL1-WDR4
Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.
N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.
METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.
Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.
N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Zhongwen (Verified Customer) (09-25-2023)
Hannah (Verified Customer) (12-05-2018)
Single specific band detected at expected size after overnight primary incubation.