MGP Monoclonal antibody

MGP Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A1C3

Cat no : 60055-1-Ig

Synonyms

GIG36, matrix Gla protein, MGLAP, MGP, NTI



Tested Applications

Positive WB detected inhuman placenta tissue, human kidney tissue, human brain tissue, human saliva, human liver cancer tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, human lung tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

60055-1-Ig targets MGP in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MGP fusion protein Ag1091
Full Name matrix Gla protein
Calculated molecular weight 103 aa, 13 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC005272
Gene symbol MGP
Gene ID (NCBI) 4256
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Protocols

Product Specific Protocols
WB protocol for MGP antibody 60055-1-IgDownload protocol
IHC protocol for MGP antibody 60055-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols