MIC10 Polyclonal antibody

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  WB analysis using 31561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31561-1-AP (MIC10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 31561-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MIC10 antibody (31561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MIC10 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31561-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C1orf151, FLJ36999, FLJ75158, UPF0327 protein C1orf151

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, L02 cells, mouse heart tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31561-1-AP targets MIC10 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIC10 fusion protein Ag34301
• Full Name: chromosome 1 open reading frame 151
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: NM_001032363
• Gene Symbol: C1orf151
• Gene ID (NCBI): 440574
• RRID: AB_3670036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5TGZ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MIC10, also known as C1orf151, MICOS10, and MINOS1, belongs to the  MICOS complex subunit Mic10 family. MIC10 is a component of the MICOS complex, which is a large protein complex located in the inner membrane of mitochondria. MIC10 plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. MIC10 is also functionally connected to the F1Fo-ATP synthase (PMID: 22114354, PMID: 28315355).