Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue|
|Positive IP detected in||HeLa cells, T-47D cells|
|Positive IHC detected in||human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14818-1-AP targets MICAL1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MICAL1 fusion protein Ag6578|
|Full Name||microtubule associated monoxygenase, calponin and LIM domain containing 1|
|Calculated molecular weight||118 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC052983|
|Gene ID (NCBI)||64780|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.
|Product Specific Protocols|
|WB protocol for MICAL1 antibody 14818-1-AP||Download protocol|
|IHC protocol for MICAL1 antibody 14818-1-AP||Download protocol|
|IF protocol for MICAL1 antibody 14818-1-AP||Download protocol|
|IP protocol for MICAL1 antibody 14818-1-AP||Download protocol|
|FC protocol for MICAL1 antibody 14818-1-AP||Download protocol|
|Click here to view our Standard Protocols|
F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.
Oxidation of F-actin controls the terminal steps of cytokinesis.
Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.
J Cell Biol
MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement
Semaphorin3a promotes advanced diabetic nephropathy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-03-2019)
Shows band at where MICAL1 is expected but is faint. This image is a merge image of the membrane exposed for 120s and a stain free image to visualize the molecular weight. Even with long exposure, the band is faint.