Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, human brain tissue, T-47D cells |
| Positive IP detected in | HeLa cells, T-47D cells |
| Positive IHC detected in | human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 16 publications below |
| IHC | See 6 publications below |
| IF | See 9 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
14818-1-AP targets MICAL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MICAL1 fusion protein Ag6578 Predict reactive species |
| Full Name | microtubule associated monoxygenase, calponin and LIM domain containing 1 |
| Calculated Molecular Weight | 118 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC052983 |
| Gene Symbol | MICAL1 |
| Gene ID (NCBI) | 64780 |
| RRID | AB_2143754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IHC protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IF protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IP protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.
| ||
Dev Cell Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling. | ||
J Cell Biol MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
| ||
Oncogene Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kincaid (Verified Customer) (06-02-2024) | Tested antibody in H4 cells. Diluted aMICAL1 1:500 in 3% BSA, diluted in TBS-tween (1%).
 |
FH Christelle (Verified Customer) (11-21-2023) | For wb: used dilution 1/1000 For IP: used 2µg of antibody
 |
FH Joleen (Verified Customer) (06-03-2019) | Shows band at where MICAL1 is expected but is faint. This image is a merge image of the membrane exposed for 120s and a stain free image to visualize the molecular weight. Even with long exposure, the band is faint.
 |
