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  • KD/KO Validated

MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 14818-1-AP



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue
Positive IP detected inHeLa cells, T-47D cells
Positive IHC detected inhuman lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14818-1-AP targets MICAL1 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICAL1 fusion protein Ag6578
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Calculated Molecular Weight 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC052983
Gene Symbol MICAL1
Gene ID (NCBI) 64780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.


Product Specific Protocols
WB protocol for MICAL1 antibody 14818-1-APDownload protocol
IHC protocol for MICAL1 antibody 14818-1-APDownload protocol
IF protocol for MICAL1 antibody 14818-1-APDownload protocol
IP protocol for MICAL1 antibody 14818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.

Authors - Fengfeng Niu
  • KO Validated

Nat Commun

Oxidation of F-actin controls the terminal steps of cytokinesis.

Authors - Stéphane Frémont
  • KD Validated

Dev Cell

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

Authors - Jimok Yoon

J Cell Biol

MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.

Authors - Chiara Galloni
  • KD Validated


Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement

Authors - Ze Zhang


Semaphorin3a promotes advanced diabetic nephropathy.

Authors - Pardeep K Aggarwal


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christelle (Verified Customer) (11-21-2023)

For wb: used dilution 1/1000 For IP: used 2µg of antibody

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Megacaryocytic cell or humans platelets
MICAL1 Antibody Western Blot,Immunoprecipitation validation (1/1000 dilution) in Megacaryocytic cell or humans platelets (Cat no:14818-1-AP)

Joleen (Verified Customer) (06-03-2019)

Shows band at where MICAL1 is expected but is faint. This image is a merge image of the membrane exposed for 120s and a stain free image to visualize the molecular weight. Even with long exposure, the band is faint.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
MICAL1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14818-1-AP)