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MITF antibody (mAb) (Clone C5.D5)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ChIP, ChIP-Seq, EMSA, ICC, IF, IP, WB

CloneNo.

C5.D5

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Cat No : 39789,39790 39789

Synonyms

MITF, Microphthalmia-associated Transcription Factor, basic helix-loop-helix, bHLH, transcription factor, antibody, monoclonal, Waardenburg syndrome type 2A, Tietz syndrome, chip, chromatin IP, chromatin immunoprecipitation, sample, chiphsabs

Validation Data Gallery

  • MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.

    MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.

  • MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human lung melanoma cell line MALME-3M and 4 ug of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human lung melanoma cell line MALME-3M and 4 ug of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Product Information

Tested Applications ChIP, ChIP-Seq, EMSA, ICC, IF, IP, WB

Applications Validated by Active Motif: ChIP: 2.5 - 10 ug per ChIP ChIP-Seq: 4 ug each This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91201.
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This MITF antibody was raised against an N-terminal fragment of human MITF.
Full Name MITF antibody (mAb) (Clone C5.D5)
Synonyms MITF, Microphthalmia-associated Transcription Factor, basic helix-loop-helix, bHLH, transcription factor, antibody, monoclonal, Waardenburg syndrome type 2A, Tietz syndrome, chip, chromatin IP, chromatin immunoprecipitation, sample, chiphsabs
Molecular weight 52-56 kDa
GenBank accession numberNP_937802
RRIDAB_2614955
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.