MLH1 Monoclonal antibody

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  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 40x lens).

MLH1 Monoclonal Antibody for IHC, ELISA

Cat No. 60822-2-Ig

Clone No.2D5A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

HNPCC2, HNPCC, hMLH1, FCC2, COCA2

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Tested Applications

• Positive IHC detected in: Human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60822-2-Ig targets MLH1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLH1 fusion protein Ag27723
• Full Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Calculated Molecular Weight: 756 aa, 85 kDa
• GenBank Accession Number: BC006850
• Gene Symbol: MLH1
• Gene ID (NCBI): 4292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P40692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer.