MMP2 Polyclonal antibody

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  WB analysis of U-87 MG using 10373-2-AP

  WB result of MMP2 antibody (10373-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-87 MG cells.

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  WB analysis using 10373-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10373-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10373-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10373-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10373-2-AP

  IP result of anti-MMP2 (IP:10373-2-AP, 4ug; Detection:10373-2-AP 1:300) with MCF-7 cells lysate 2400 ug.

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  IHC staining of human prostate cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10373-2-AP (MMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10373-2-AP (MMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10373-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (10373-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

MMP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10373-2-AP

Publications(939)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Matrix metalloproteinase 2, Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase

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Tested Applications

• Positive WB detected in: HT-1080 cells, MCF-7 cells, U-87 MG cells, HepG2 cells, U-251 cells, J774A.1 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human gliomas tissue, human placenta tissue, human prostate cancer tissue, human heart tissue, human oesophagus tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

In sample preparation, the extracellular matrix needs to be included

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 850 publications below
• IHC: See 124 publications below
• IF: See 45 publications below
• IP: See 1 publications below

Product Information

10373-2-AP targets MMP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, bovine, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP2 fusion protein Ag0549
• Full Name: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 62-72 kDa
• GenBank Accession Number: BC002576
• Gene Symbol: MMP2
• Gene ID (NCBI): 4313
• RRID: AB_2250823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08253
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

Publications

Species	Application	Title
mouse	IHC	J Thromb Haemost; Pharmacological reduction of coagulation factor XI reduces macrophage accumulation and accelerates deep vein thrombosis resolution in a mouse model of venous thrombosis.; Authors - Kelley R Jordan
mouse	IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
mouse	WB	Circ Res; Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction.; Authors - Guizhen Zhao
human	IHC	J Thorac Oncol; Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling.; Authors - Baoping Cao
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
human	WB	Nat Commun; Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.; Authors - Shu-Chen Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (07-11-2022)

it doesn't seem that the antibody recognized anything specific on this blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: mouse macrophage cell line J774

MMP2 Antibody Western Blot validation (1:600 dilution) in mouse macrophage cell line J774 (Cat no:10373-2-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

Not good for wb, lots of bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP2 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:10373-2-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this on mouse aneurysm sectioning for IHC. It works very well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: aorta

FH

Lucy (Verified Customer) (03-08-2019)

Reacts to human MMP2 but not human MMP9

• Applications: Western Blot,

MMP2 Antibody Western Blot, validation ( dilution) in (Cat no:10373-2-AP)