MMP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

603

Reviews (4)

Cat no : 10373-2-AP

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1

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Validation Data Gallery

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  WB analysis using 10373-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 10373-2-AP

  WB result of MMP2 antibody (10373-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells.

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  WB analysis of HepG2 using 10373-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10373-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10373-2-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10373-2-AP

  IP result of anti-MMP2 (IP:10373-2-AP, 4ug; Detection:10373-2-AP 1:300) with MCF-7 cells lysate 2400 ug.

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  IHC staining of human gliomas using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human oesophagus using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10373-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10373-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10373-2-AP (MMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: U-251 cells, MCF-7 cells, HepG2 cells, HT-1080 cells, U-87 MG cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human gliomas tissue, human heart tissue, human breast cancer tissue, human placenta tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 546 publications below
• IHC: See 75 publications below
• IF: See 17 publications below
• IP: See 1 publications below

Product Information

10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine, fish, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP2 fusion protein Ag0549
• Full Name: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Calculated molecular weight: 72 kDa
• Observed molecular weight: 62-72 kDa
• GenBank accession number: BC002576
• Gene symbol: MMP2
• Gene ID (NCBI): 4313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

Publications

Species	Application	Title
human	WB	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen
mouse	WB	Dose Response; Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.; Authors - Xin Jiang
human	WB	Exp Ther Med; Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.; Authors - Tangshan Zhang
	WB	Int J Mol Med; miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.; Authors - Tao Han
human	WB	Int J Oncol; 25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.; Authors - Saisai Wang
human	WB	Lab Invest; Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis.; Authors - Longfei Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (07-11-2022)

it doesn't seem that the antibody recognized anything specific on this blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: mouse macrophage cell line J774

MMP2 Antibody Western Blot validation (1:600 dilution) in mouse macrophage cell line J774 (Cat no:10373-2-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

Not good for wb, lots of bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP2 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:10373-2-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this on mouse aneurysm sectioning for IHC. It works very well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: aorta

FH

Lucy (Verified Customer) (03-08-2019)

Reacts to human MMP2 but not human MMP9

• Applications: Western Blot,

MMP2 Antibody Western Blot, validation ( dilution) in (Cat no:10373-2-AP)

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