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MMP2 Antibody 170 Publications

Rabbit Polyclonal| Catalog number: 10373-2-AP

Featured Product KD/KO validated

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

MMP2 Antibody WB MCF-7 cells 10373-2-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP(MMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MMP2 Antibody WB U-251 cells 10373-2-AP

WB result of MMP2 antibody (10373-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells

MMP2 Antibody IP MCF-7 cells 10373-2-AP

IP result of anti-MMP2(IP:10373-2-AP, 4ug; Detection:10373-2-AP 1:300) with MCF-7 cells lysate 2400 ug

MMP2 Antibody IHC human gliomas tissue 10373-2-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP( MMP2 Antibody) at dilution of 1:200 (under 10x lens).

MMP2 Antibody IHC human gliomas tissue 10373-2-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP( MMP2 Antibody) at dilution of 1:200 (under 40x lens).

MMP2 Antibody IF MCF-7 cells 10373-2-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 10373-2-AP(MMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, zebrafish, Astragalus membranaceus, mousr, rabbit
Positive WB detected in:
MCF7 cells, MCF-7 cells, A375 cells, MDA-MB-453s cells, mouse liver tissue, HepG2 cells, rat heart tissue, HT-1080 cells, A431 cells, mouse heart tissue, mouse lung tissue, U-251 cells, SGC-7901 cells
Positive IP detected in:
MCF-7 cells, NIH/3T3 cells
Positive IHC detected in:
human gliomas tissue, human placenta tissue, human oesophagus tissue, human heart tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MMP2 fusion protein Ag0549
Full name:
matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Calculated molecular weight:
72 kDa
Observed molecular weight:
72 kDa, 60 kDa
GenBank accession number:
BC002576
Gene ID (NCBI):
4313
Gene symbol
MMP2
Synonyms
72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1

Background

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697).


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