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MMP2 Polyclonal antibody

MMP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10373-2-AP

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1



Tested Applications

Positive WB detected inMCF-7 cells, mouse liver tissue, mouse lung tissue, SGC-7901 cells, U-251 cells, HepG2 cells, rat heart tissue, mouse heart tissue, A431 cells, HT-1080 cells, A375 cells, MDA-MB-453s cells
Positive IP detected inMCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman gliomas tissue, human heart tissue, mouse liver tissue, human oesophagus tissue, human breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Astragalus membranaceus, human, mouse, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP2 fusion protein Ag0549
Full Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Calculated molecular weight 72 kDa
Observed molecular weight53-72 kDa
GenBank accession numberBC002576
Gene symbol MMP2
Gene ID (NCBI) 4313
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697).

Protocols

Product Specific Protocols
WB protocol for MMP2 antibody 10373-2-APDownload protocol
IHC protocol for MMP2 antibody 10373-2-APDownload protocol
IF protocol for MMP2 antibody 10373-2-APDownload protocol
IP protocol for MMP2 antibody 10373-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.

Authors - Erfei Chen
mouseWB

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
humanWB

Exp Ther Med

Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.

Authors - Tangshan Zhang
WB

Int J Mol Med

miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.

Authors - Tao Han
humanWB

Int J Oncol

25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.

Authors - Saisai Wang
humanWB

Lab Invest

Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis.

Authors - Longfei Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this on mouse aneurysm sectioning for IHC. It works very well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: aorta
FH

Lucy (Verified Customer) (03-08-2019)

Reacts to human MMP2 but not human MMP9

  • Applications: Western Blot,
MMP2 Antibody Western Blot, validation ( dilution) in (Cat no:10373-2-AP)