|Positive WB detected in||MCF-7 cells, mouse liver tissue, mouse lung tissue, SGC-7901 cells, U-251 cells, HepG2 cells, rat heart tissue, mouse heart tissue, A431 cells, HT-1080 cells, A375 cells, MDA-MB-453s cells|
|Positive IP detected in||MCF-7 cells, NIH/3T3 cells|
|Positive IHC detected in||human gliomas tissue, human heart tissue, mouse liver tissue, human oesophagus tissue, human breast cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Astragalus membranaceus, human, mouse, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP2 fusion protein Ag0549|
|Full Name||matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||53-72 kDa|
|GenBank accession number||BC002576|
|Gene ID (NCBI)||4313|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697).
|Product Specific Protocols|
|WB protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IHC protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IF protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IP protocol for MMP2 antibody 10373-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
I am working on aneurysm study and use this on mouse aneurysm sectioning for IHC. It works very well.
Lucy (Verified Customer) (03-08-2019)
Reacts to human MMP2 but not human MMP9