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MMP7 Polyclonal antibody

MMP7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10374-2-AP

Synonyms

Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase



Tested Applications

Positive WB detected inCOLO 320 cells, human placenta tissue, mouse lung tissue, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10374-2-AP targets MMP7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP7 fusion protein Ag0550
Full Name matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight 29 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC003635
Gene symbol MMP7
Gene ID (NCBI) 4316
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Protocols

Product Specific Protocols
WB protocol for MMP7 antibody 10374-2-APDownload protocol
IHC protocol for MMP7 antibody 10374-2-APDownload protocol
IF protocol for MMP7 antibody 10374-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Inhibition of fatty acid synthesis arrests colorectal neoplasm growth and metastasis: Anti-cancer therapeutical effects of natural cyclopeptide RA-XII.

Authors - Yurong Wang
humanIHC

Oncogene

AKIP1 promotes early recurrence of hepatocellular carcinoma through activating the Wnt/β-catenin/CBP signaling pathway.

Authors - Yanmei Cui
humanIHC

Antioxidants (Basel)

Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models.

Authors - Athanasios Karapetsas
humanWB

Technol Cancer Res Treat

Nicotine Induces Progressive Properties of Lung Adenocarcinoma A549 Cells by Inhibiting Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Plasma Membrane Localization.

Authors - Hui Li
humanWB

Biochem Biophys Res Commun

Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.

Authors - Xiao-Xiao He
human,mouseWB

J Cell Mol Med

Cyclin G2 regulates canonical Wnt signalling via interaction with Dapper1 to attenuate tubulointerstitial fibrosis in diabetic nephropathy.

Authors - Chenyang Zhao