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MMP7 Antibody 29 Publications

Rabbit Polyclonal| Catalog number: 10374-2-AP

Featured Product KD/KO validated

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Con: 55 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

MMP7 Antibody WB COLO 320 cells 10374-2-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MMP7 Antibody IHC human pancreas cancer tissue 10374-2-AP

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP( MMP7 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MMP7 Antibody IHC human pancreas cancer tissue 10374-2-AP

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP( MMP7 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MMP7 Antibody IF human pancreas cancer tissue 10374-2-AP

Immunofluorescent analysis of ( 4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MMP7 Antibody IF human pancreas cancer tissue 10374-2-AP

Immunofluorescent analysis of ( 4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
COLO 320 cells, human placenta tissue, mouse lung tissue, NIH/3T3 cells
Positive IHC detected in:
human pancreas cancer tissue, human stomach cancer tissue, human stomach tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human pancreas cancer tissue
Recommended dilution:
WB : 1:300-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MMP7 fusion protein Ag0550
Full name:
matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight:
29 kDa
Observed molecular weight:
28-30 kDa
GenBank accession number:
BC003635
Gene ID (NCBI):
4316
Gene symbol
MMP7
Synonyms
Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase

Background

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. The gene encoding MMP-7 is part of a cluster of MMP genes which localize to chromosome 11q22.3.


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