MMP9 (Middle) Polyclonal antibody

MMP9 (Middle) Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 27306-1-AP

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9

---

Validation Data Gallery

• 

  WB analysis of human saliva using 27306-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human saliva using 27306-1-AP

  IP result of anti-MMP9 (Middle)(IP:27306-1-AP, 4ug; Detection:27306-1-AP 1:300) with human saliva lysate 800 ug.

• 

  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human saliva tissue
• Positive IP detected in: human saliva tissue
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 14 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 27306-1-AP is MMP9 (Middle) Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP9 (Middle) fusion protein Ag26132
• Full Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Calculated molecular weight: 707 aa, 78 kDa
• Observed molecular weight: 92 kDa
• GenBank accession number: BC006093
• Gene symbol: MMP9
• Gene ID (NCBI): 4318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	WB	Med Sci Monit; Integrin Subunit Alpha 5 (ITGA5) Gene Circular RNA Sponges microRNA-107 in Colorectal Carcinoma Cells and Tissues and Regulates the Expression of the Forkhead Box J3 (FOXJ3) Gene.; Authors - Guoqiang Huang
human	WB	Biomed Res Int; miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1.; Authors - Dan Li
human	WB	Chin J Nat Med; Therapeutic effect of neohesperidin on TNF-α-stimulated human rheumatoid arthritis fibroblast-like synoviocytes; Authors - WANG Xiao-He
human	WB	Oncol Lett; Zinc-finger protein 750 mitigates malignant biological behavior of oral CSC-like cells enriched from parental CAL-27 cells.; Authors - Cong Xu
human	WB	Oncol Lett; The TGF-β1/COX-2-dependant pathway serves a key role in the generation of OKC-induced M2-polarized macrophage-like cells and angiogenesis.; Authors - Gang Cheng
human	WB	Cell Biol Int; UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.; Authors - Zhi Cui