MMP9 (Middle) Polyclonal antibody

MMP9 (Middle) Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 27306-1-AP

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9

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Validation Data Gallery

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  WB analysis of human saliva using 27306-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human saliva using 27306-1-AP

  IP result of anti-MMP9 (Middle)(IP:27306-1-AP, 4ug; Detection:27306-1-AP 1:300) with human saliva lysate 800 ug.

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  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27306-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human saliva tissue
• Positive IP detected in: human saliva tissue
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 27306-1-AP is MMP9 (Middle) Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP9 (Middle) fusion protein Ag26132
• Full Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Calculated molecular weight: 707 aa, 78 kDa
• Observed molecular weight: 92 kDa
• GenBank accession number: BC006093
• Gene symbol: MMP9
• Gene ID (NCBI): 4318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human	WB	Front Mol Biosci; Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer.; Authors - Liang Huang
human	WB	Front Mol Biosci; miR-193a-3p Mediates Placenta Accreta Spectrum Development by Targeting EFNB2 via Epithelial-Mesenchymal Transition Pathway Under Decidua Defect Conditions.; Authors - Na Li
human	WB	Front Mol Biosci; CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.; Authors - Fan Feng
human	WB	Int Immunopharmacol; IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.; Authors - Yachun Li
rat	WB	Life Sci; Differentially expressed genes induced by β-caryophyllene in a rat model of cerebral ischemia-reperfusion injury.; Authors - Shengwei Liu