MMP9 (Middle) Polyclonal antibody

MMP9 (Middle) Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27306-1-AP

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP9 (Middle)



Tested Applications

Positive WB detected inhuman saliva tissue
Positive IP detected inhuman saliva tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27306-1-AP targets MMP9 (Middle) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP9 (Middle) fusion protein Ag26132
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC006093
Gene symbol MMP9
Gene ID (NCBI) 4318
RRIDAB_2880837
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Protocols

Product Specific Protocols
WB protocol for MMP9 (Middle) antibody 27306-1-APDownload protocol
IHC protocol for MMP9 (Middle) antibody 27306-1-APDownload protocol
IP protocol for MMP9 (Middle) antibody 27306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Mol Sci

Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion

Authors - Tianxiang Xia
mouseWB

Front Endocrinol (Lausanne)

Nicotinamide mononucleotide attenuates HIF-1α activation and fibrosis in hypoxic adipose tissue via NAD+/SIRT1 axis

Authors - Keke Wu
humanWB

Int J Biol Sci

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

Authors - Jiyu Miao
humanWB

Front Mol Biosci

Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer.

Authors - Liang Huang
humanWB

Front Mol Biosci

miR-193a-3p Mediates Placenta Accreta Spectrum Development by Targeting EFNB2 via Epithelial-Mesenchymal Transition Pathway Under Decidua Defect Conditions.

Authors - Na Li
humanWB

Front Mol Biosci

CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.

Authors - Fan Feng