MPZ / P0 Polyclonal antibody

MPZ / P0 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10572-1-AP

Synonyms

CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, MPP, MPZ, MPZ / P0, Myelin peripheral protein, Myelin protein P0, myelin protein zero, P0



Tested Applications

Positive WB detected inSchwann cells
Positive IF detected inmyelinating SCs cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10572-1-AP targets MPZ / P0 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityfrog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPZ / P0 fusion protein Ag0848
Full Name myelin protein zero
Calculated molecular weight 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC006491
Gene symbol MPZ
Gene ID (NCBI) 4359
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MPZ (myelin protein zero), also known as P0, a transmembrane glycoprotein (~30 kDa), is a member of the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in formation and maintenance of compact myelin, and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ, and can be used as a marker of myelinating Schwann cells.

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.

Authors - Chunyang Xu
human,rat,frogWB

Neurochem Res

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.

Authors - Yoshihide Yamaguchi
humanIHC

PLoS One

Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy.

Authors - Andrew J Schwab
mouse,ratWB

Mol Cell Neurosci

MMPs initiate Schwann cell-mediated MBP degradation and mechanical nociception after nerve damage.

Authors - Kobayashi Hideo H
mouseWB

Front Cell Neurosci

Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR.

Authors - Christoph Eckharter
ratIF

J Neuropathol Exp Neurol

Matrix metalloproteinase inhibition enhances the rate of nerve regeneration in vivo by promoting dedifferentiation and mitosis of supporting schwann cells.

Authors - Liu Huaqing H