|Positive WB detected in||HEK-293 cells, HEK-293|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
18120-1-AP targets MSH6 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MSH6 fusion protein Ag12645|
|Full Name||mutS homolog 6 (E. coli)|
|Calculated molecular weight||153 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||BC004246|
|Gene ID (NCBI)||2956|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.
|Product Specific Protocols|
|WB protocol for MSH6 antibody 18120-1-AP||Download protocol|
|IHC protocol for MSH6 antibody 18120-1-AP||Download protocol|
|IF protocol for MSH6 antibody 18120-1-AP||Download protocol|
|IP protocol for MSH6 antibody 18120-1-AP||Download protocol|
|FC protocol for MSH6 antibody 18120-1-AP||Download protocol|
|Click here to view our Standard Protocols|
DNA Cell Biol
MSH6 Aggravates the Hypoxic Microenvironment via Regulating HIF1A to Promote the Metastasis of Glioblastoma Multiforme.
Targeted silencing of SOX2 by an artificial transcription factor showed antitumor effect in lung and esophageal squamous cell carcinoma.
CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.