Methylmalonyl Coenzyme A mutase/MUT Polyclonal antibody

• 

  WB analysis using 17034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17034-1-AP

  IP result of anti-Methylmalonyl Coenzyme A mutase/MUT (IP:17034-1-AP, 4ug; Detection:17034-1-AP 1:500) with HepG2 cells lysate 1800ug.

• 

  IHC staining of human liver cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Methylmalonyl Coenzyme A mutase/MUT Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17034-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

17034-1-AP targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Full Name: methylmalonyl Coenzyme A mutase
• Calculated Molecular Weight: 750 aa, 83 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC016282
• Gene Symbol: MUT
• Gene ID (NCBI): 4594
• RRID: AB_2147263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22033
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.

Publications

Species	Application	Title
mouse	IHC	Hepatology; Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia.; Authors - Randy J Chandler
mouse,human	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse	IHC	PLoS One; Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia; Authors - Shengwen Zhang
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell
human	WB	Biochim Biophys Acta Mol Basis Dis; ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis; Authors - Lan Wang
human	WB,IHC	Am J Pathol; Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis; Authors - Kai Xu ; KD Validated