c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IHC, FC, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

3D9C12

Cat no : 67447-1-Ig

Synonyms

bHLHe39, c Myc, c-MYC, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64



Tested Applications

Positive WB detected inMDA-MB-231 cells, HT-29 cells
Positive IHC detected inhuman colon cancer tissue, human tonsillitis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67447-1-Ig targets c-MYC in WB, IP, IHC, FC, CoIP,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-MYC fusion protein Ag17519
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000141
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 67447-1-IgDownload protocol
IHC protocol for c-MYC antibody 67447-1-IgDownload protocol
FC protocol for c-MYC antibody 67447-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anticancer Drugs

Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.

Authors - Chenxue Gong
humanWB

Oncol Lett

DEK is highly expressed in breast cancer and is associated with malignant phenotype and progression.

Authors - Mai-Qing Yang
humanWB

Parasit Vectors

The NF-κB signalling pathway and TM7SF3 contribute to liver fibrosis caused by secreted phospholipase A2 of Clonorchis sinensis.

Authors - Yin-Juan Wu
WB,CoIP

Vet Microbiol

PRV-encoded UL13 protein kinase acts as an antagonist of innate immunity by targeting IRF3-signaling pathways.

Authors - Lin Lv
WB

Front Genet

Epilepsy-Associated UBE3A Deficiency Downregulates Retinoic Acid Signalling Pathway.

Authors - Meimiao Fang
humanWB

Ann Transl Med

LncRNA ABCC6P1 promotes proliferation and migration of papillary thyroid cancer cells via Wnt/β-catenin signaling pathway.

Authors - Yue Guan