Tested Applications
| Positive WB detected in | MDA-MB-231 cells, HT-29 cells, 4T1 cells, PC-3 cells, K-562 cells, HL-60 cells, Raji cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, mouse brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | K-562 cells, NCCIT cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 116 publications below |
| IHC | See 26 publications below |
| IF | See 5 publications below |
| IP | See 9 publications below |
| CoIP | See 6 publications below |
| ChIP | See 1 publications below |
Product Information
67447-1-Ig targets c-MYC in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bacillus subtilis |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17519 Product name: Recombinant human MYC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA Predict reactive species |
| Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC000141 |
| Gene Symbol | MYC |
| Gene ID (NCBI) | 4609 |
| RRID | AB_2882681 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01106 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYC contains a basic helix-loop-helix (bHLH) domain. This protein is a multifunctional nuclear phosphoprotein, which plays a role in cell cycle process, apoptosis and cell transformation. As a transcription factor, it regulates the transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds to DNA in a nonspecific way, but also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutation, overexpression, rearrangement and translocation of this gene are related to many hematopoietic tumors, leukemia and lymphoma (including Burkitt lymphoma). The antibody is a rabbit polyclonal antibody against human MYC recombinant protein. The 50kDa band recognized by antibody is the natural form of MYC, while other bands between 60 and 70 kDa are the phosphorylated form of MYC (PMID: 12189186). The latest research reveals a new key mechanism of MYC protein, which is independent of its classical transcriptional regulation function. It was found that under the stress of transcription, MYC was polymerized through its specific RNA binding domain (RBRIII), and exocrine was targeted at the nuclear R loop and double-stranded RNA. This process can effectively eliminate the immunogenic RNA-DNA hybrid from R-loop, thus preventing it from activating TLR3-TBK1 natural immune signaling pathway. This mechanism is very important for MYC to maintain tumor growth and achieve immune escape in vivo. (PMID: 41576951)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for c-MYC antibody 67447-1-Ig | Download protocol |
| IHC protocol for c-MYC antibody 67447-1-Ig | Download protocol |
| WB protocol for c-MYC antibody 67447-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Drug Resist Updat Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression
| ||
Nat Commun ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer | ||
Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. | ||
Acta Pharm Sin B Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors. | ||
Sci Adv AKR1B10 dictates c-Myc stability to suppress colorectal cancer metastasis via PP2A nitration |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tanis (Verified Customer) (03-15-2022) | Antibody was strong, clean, and specific in our application.
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