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  • KD/KO Validated

MYH6 Polyclonal antibody

MYH6 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 22281-1-AP


alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6

Tested Applications

Positive WB detected inmouse heart tissue, rat heart
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22281-1-AP targets MYH6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 6, cardiac muscle, alpha
Calculated Molecular Weight 224 kDa
Observed Molecular Weight 200-220 kDa
GenBank Accession NumberNM_002471
Gene Symbol MYH6
Gene ID (NCBI) 4624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)


Product Specific Protocols
WB protocol for MYH6 antibody 22281-1-APDownload protocol
IHC protocol for MYH6 antibody 22281-1-APDownload protocol
IF protocol for MYH6 antibody 22281-1-APDownload protocol
IP protocol for MYH6 antibody 22281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.

Authors - John T Hinson

Dev Cell

Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.

Authors - Anant Chopra


Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp

Authors - Abigail C Neininger-Castro
  • KD Validated

Stem Cell Reports

Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development.

Authors - Silvia Mazzotta

J Pathol

Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.

Authors - Chen Ni

Int J Mol Sci

Circular RNA circIGF2BP3 Promotes the Proliferation and Differentiation of Chicken Primary Myoblasts

Authors - Xiaotong Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hua (Verified Customer) (01-20-2023)

Very good antibody. Strong signal and clean background in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Mouse heart
MYH6 Antibody Western Blot validation (1:8000 dilution) in Mouse heart (Cat no:22281-1-AP)

Jane (Verified Customer) (02-03-2022)

one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart tissue

Jing (Verified Customer) (02-08-2021)

good antibody, clear sigle band around 260KD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse heart