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MYH6 Polyclonal antibody
MYH6 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (2)
WB, IHC, IF, ELISA
Cat no : 22281-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat heart|
|Positive IHC detected in||human heart tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 13 publications below|
|IHC||See 4 publications below|
|IF||See 8 publications below|
22281-1-AP targets MYH6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, heavy chain 6, cardiac muscle, alpha|
|Calculated molecular weight||224 kDa|
|Observed molecular weight||200-220 kDa|
|GenBank accession number||NM_002471|
|Gene ID (NCBI)||4624|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
|Product Specific Protocols|
|WB protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IHC protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IF protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IP protocol for MYH6 antibody 22281-1-AP||Download protocol|
|Click here to view our Standard Protocols|
HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.
Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.
Stem Cell Reports
Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development.
Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.
Differential roles of YTHDF1 and YTHDF3 in embryonic stem cell-derived cardiomyocyte differentiation.
Ecotoxicol Environ Saf
Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (01-20-2023)
Very good antibody. Strong signal and clean background in WB.
Jane (Verified Customer) (02-03-2022)
one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.
Jing (Verified Customer) (02-08-2021)
good antibody, clear sigle band around 260KD.