Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat heart|
|Positive IHC detected in||human heart tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22281-1-AP targets MYH6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, heavy chain 6, cardiac muscle, alpha|
|Calculated molecular weight||224 kDa|
|Observed molecular weight||200-220 kDa|
|GenBank accession number||NM_002471|
|Gene ID (NCBI)||4624|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
|Product Specific Protocols|
|WB protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IHC protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IF protocol for MYH6 antibody 22281-1-AP||Download protocol|
|IP protocol for MYH6 antibody 22281-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (01-20-2023)
Very good antibody. Strong signal and clean background in WB.
Jane (Verified Customer) (02-03-2022)
one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.
Jing (Verified Customer) (02-08-2021)
good antibody, clear sigle band around 260KD.