MYH6 Polyclonal antibody

MYH6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 22281-1-AP


alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6

Tested Applications

Positive WB detected inmouse heart tissue, rat heart
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22281-1-AP targets MYH6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 6, cardiac muscle, alpha
Calculated molecular weight 224 kDa
Observed molecular weight 200-220 kDa
GenBank accession numberNM_002471
Gene symbol MYH6
Gene ID (NCBI) 4624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)


Product Specific Protocols
WB protocol for MYH6 antibody 22281-1-APDownload protocol
IHC protocol for MYH6 antibody 22281-1-APDownload protocol
IF protocol for MYH6 antibody 22281-1-APDownload protocol
IP protocol for MYH6 antibody 22281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun

Stem Cell Rev Rep

MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.

Authors - Xiangyu Zhang

Front Public Health

Examination of the Effect of a 50-Hz Electromagnetic Field at 500 μT on Parameters Related With the Cardiovascular System in Rats.

Authors - Yemao Zhang

BMC Genet

Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression.

Authors - I-Hsuan Lin

J Cardiovasc Pharmacol

Senolytic Agent Navitoclax Inhibits Angiotensin II-Induced Heart Failure in Mice.

Authors - Kangni Jia

DNA Cell Biol

Asporin is a Potential Promising Biomarker for Common Heart Failure.

Authors - Kai Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jane (Verified Customer) (02-03-2022)

one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart tissue

Jing (Verified Customer) (02-08-2021)

good antibody, clear sigle band around 260KD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse heart