Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 33 publications below |
| IHC | See 5 publications below |
| IF | See 18 publications below |
Product Information
22281-1-AP targets MYH6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 6, cardiac muscle, alpha |
| Calculated Molecular Weight | 224 kDa |
| Observed Molecular Weight | 200-220 kDa |
| GenBank Accession Number | NM_002471 |
| Gene Symbol | MYH6 |
| Gene ID (NCBI) | 4624 |
| RRID | AB_2736822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH6 antibody 22281-1-AP | Download protocol |
| IHC protocol for MYH6 antibody 22281-1-AP | Download protocol |
| IP protocol for MYH6 antibody 22281-1-AP | Download protocol |
| WB protocol for MYH6 antibody 22281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. | ||
Dev Cell Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions. | ||
Elife Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp
| ||
Stem Cell Reports Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development. | ||
J Pathol Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlène (Verified Customer) (04-16-2025) | The antibody is working well on our samples
 |
FH Hua (Verified Customer) (01-20-2023) | Very good antibody. Strong signal and clean background in WB.
 |
FH Jane (Verified Customer) (02-03-2022) | one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.
|
FH Jing (Verified Customer) (02-08-2021) | good antibody, clear sigle band around 260KD.
|
