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  • KD/KO Validated

MYO18A Polyclonal antibody

MYO18A Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14611-1-AP

Synonyms

CD245, KIAA0216, MAJN, MYO18A, Myosin containing a PDZ domain, myosin XVIIIA, MYSPDZ, SPR210



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14611-1-AP targets MYO18A in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO18A fusion protein Ag6185
Full Name myosin XVIIIA
Calculated molecular weight 233 kDa
Observed molecular weight 230 kDa, 190 kDa
GenBank accession numberBC039612
Gene symbol MYO18A
Gene ID (NCBI) 399687
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for MYO18A antibody 14611-1-APDownload protocol
IP protocol for MYO18A antibody 14611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Authors - Sophie Uzureau
mouseFC

Antiviral Res

GM-CSF modulates pulmonary resistance to influenza A infection.

Authors - Sever-Chroneos Zvjezdana Z
mouseWB

J Biol Chem

A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.

Authors - Markus Horsthemke
IF

J Cell Sci

Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.

Authors - Helena Sofia Domingues
mouseWB

J Biol Chem

Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.

Authors - Sever-Chroneos Zvjezdana Z
  • KD Validated
humanWB

Diabetes

Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.

Authors - Duc T Tran