MYPT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 22117-1-AP

Synonyms

MBS, MYPT1, PPP1R12A

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Validation Data Gallery

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  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22117-1-AP

  WB result of MYPT1 antibody (22117-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYPT1 transfected HEK-293 cells.

• 

  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22117-1-AP

  IP result of anti-MYPT1 (IP:22117-1-AP, 4ug; Detection:22117-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.

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  IHC staining of human heart using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22117-1-AP (MYPT1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22117-1-AP (MYPT1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human gliomas using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 22117-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, C2C12 cells, C6 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue, human gliomas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

22117-1-AP targets MYPT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYPT1 fusion protein Ag17496
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Calculated Molecular Weight: 1030 aa, 115 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC111752
• Gene Symbol: PPP1R12A
• Gene ID (NCBI): 4659
• RRID: AB_11183753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization. Several transcript variants encoding different isoforms have been found for this gene.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization.; Authors - Yan Qi
rat	WB	Sci Rep; MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.; Authors - Yan Zeng
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
rat	WB	Front Pharmacol; Madagascine Induces Vasodilatation via Activation of AMPK.; Authors - Dapeng Chen
pig	WB, IF	Cells; Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.; Authors - Sylvia Fenosoa Rasamizafy ; KD Validated
human	WB	Food Funct; Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.; Authors - Luqing Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siddharth (Verified Customer) (07-28-2023)

Good for western blotting. Band detected around 120 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: M2-10B4 cell-line (mouse bone marrow stromal cells)

FH

Claudia (Verified Customer) (10-19-2021)

Unmasking microwave: potencia medium, ph6, 10 min., cool 35 min., room temperature.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Porcine coronaryarteries on paraffin-embedded sections