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  • KD/KO Validated

MYPT1 Polyclonal antibody

MYPT1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22117-1-AP

Synonyms

MBS, MYPT1, PPP1R12A



Tested Applications

Positive WB detected inHEK-293 cells, C2C12 cells, HeLa cells, Jurkat cells, MCF-7 cells, C6 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human gliomas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22117-1-AP is MYPT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYPT1 fusion protein Ag17496
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Calculated molecular weight 1030 aa, 115 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC111752
Gene symbol PPP1R12A
Gene ID (NCBI) 4659
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization. Several transcript variants encoding different isoforms have been found for this gene.

Protocols

Product Specific Protocols
WB protocol for MYPT1 antibody 22117-1-APDownload protocol
IHC protocol for MYPT1 antibody 22117-1-APDownload protocol
IF protocol for MYPT1 antibody 22117-1-APDownload protocol
IP protocol for MYPT1 antibody 22117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization.

Authors - Yan Qi
humanIHC

J Cancer

MicroRNA-455-5p Contributes to Cholangiocarcinoma Growth and Mediates Galangin's Anti-Tumor Effects.

Authors - Xu Deng
mouseIHC

Sci Rep

Smad4 regulates the nuclear translocation of Nkx2-5 in cardiac differentiation.

Authors - Wenyu Hu
pigWB, IF

Cells

Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.

Authors - Sylvia Fenosoa Rasamizafy
  • KD Validated
ratWB

Front Pharmacol

Madagascine Induces Vasodilatation via Activation of AMPK.

Authors - Dapeng Chen
mouseWB

Sci Rep

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Authors - Dongli Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudia (Verified Customer) (10-19-2021)

Unmasking microwave: potencia medium, ph6, 10 min., cool 35 min., room temperature.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Porcine coronaryarteries on paraffin-embedded sections