Recombinant Mouse 5'-Nucleotidase/CD73 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1389
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse 5'-Nucleotidase/CD73 protein Trp29-Ser551 (Accession# Q61503) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 23959 |
| Accession | Q61503 |
| PredictedSize | 84.3 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues. CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates. In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells. CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression.
References:
1. Colgan, Sean P et al. Purinergic signalling vol. 2,2 (2006): 351-60. 2. Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55. 3. Resta, R et al. Immunological reviews vol. 161 (1998): 95-109. 4. Thomson, L F et al. Tissue antigens vol. 35,1 (1990): 9-19. 5. Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55.