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Recombinant Mouse ACBP/DBI protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2462

Validation Data Gallery

  • Purity of Recombinant Mouse ACBP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse ACBP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse ACBP/DBI was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse ACBP/DBI was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse ACBP protein Met1-Ile87 (Accession# P31786) with a rabbit IgG Fc tag at the N-terminus.
GeneID 13167
Accession P31786
PredictedSize 37.2 kDa
SDS-PAGE 35-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Acyl coenzyme A binding protein (ACBP), also known as diazepam-binding inhibitor (DBI), is a phylogenetically ancient protein present in some eubacteria and the entire eukaryotic radiation. In several eukaryotic phyla, ACBP/DBI transcends its intracellular function in fatty acid metabolism because it can be released into the extracellular space.Indeed, autophagy is tied to the atypical secretion of this leaderless protein that is predominantly present in the cytosol of nucleated cells. Once released into the extracellular space, ACBP/DBI then acts on gamma-aminobutyric acid (GABA) receptors to inhibit autophagy via autocrine, paracrine, and neuroendocrine pathways.

References:

1.Montégut, Léa et al. (2023) Aging Cell.22(9):e13910. 
2.Motiño, Omar et al. (2022) Proc Natl Acad Sci U S A. 119(41):e2207344119. 
3.Alquier, Thierry et al. (2021) Trends Endocrinol Metab. 32(11):890-903.