Mouse Albumin ELISA Kit
Cat no : KE10117
Synonyms
Alb,Alb 1,Alb1,albumin
Validation Data Gallery
Product Information
KE10117 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Albumin. The assay recognizes mouse Albumin. An antibody specific for mouse Albumin has been pre-coated onto the microwells. The mouse Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Albumin is added to detect the captured mouse Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Albumin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11657 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 107% | 80%-126% |
| Urine | 96% | 86%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 748.6 | 43.1 | 5.8 |
| 2 | 20 | 172.6 | 3.4 | 1.9 |
| 3 | 20 | 39.1 | 1.5 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 737.3 | 64.6 | 8.8 |
| 2 | 24 | 174.4 | 13.3 | 7.6 |
| 3 | 24 | 44.4 | 4.2 | 9.4 |
Background Information
Albumin is one of the main proteins in animal species, which plays a decisive role in the transport of various ions and in maintaining the colloidal osmotic pressure of the blood. Albumin is able to bind to almost all known drugs, as well as many nutraceuticals and toxic substances, largely determining their pharmaco-and toxicokinetics. Albumin is not only passive, but also an active participant of pharmacokinetic and toxicokinetic processes, possessing a number of enzymatic activities. Numerous experiments have shown esterase or pseudoesterase activity of albumin towards a number of endogeneous and exogeneous esters. Due to the free thiol group of Cys34, albumin can serve as a trap for reactive oxygen and nitrogen species, thus participating in redox processes. Glycated albumin makes a significant contribution to the pathogenesis of diabetes and other diseases. The interaction of albumin with blood cells, blood vessels and tissue cells outside the vascular bed is of great importance. Interactions with endothelial glycocalyx and vascular endothelial cells largely determine the integrative role of albumin.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alb,Alb 1,Alb1,albumin |