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Recombinant Mouse ANGPTL3 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0662

Validation Data Gallery

  • Purity of Recombinant Human ANGPTL3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ANGPTL3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse ANGPTL3 protein Ser17-Thr455 (Accession# Q9R182) with a His tag at the C-terminus.
GeneID 30924
Accession Q9R182
PredictedSize 52.3 kDa
SDS-PAGE 60-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases. Overexpression of Angptl3 or intravenous injection of the purified protein in KK/San mice elicited a marked increase in circulating plasma lipid levels. It suggests that Angptl3 regulates lipid metabolism in mice.

References:

1.Pei-Yi Chen. et al. (2021) Int J Mol Sci. 7;22(14):7310.
2.Shuang Jiang.et al. (2019) J Drug Target.27(8):876-884.
3.Ryuta Koishi. et al. (2002) Nat Genet.30(2):151-7.
4.Anna Tikka. et al. (2016) Endocrine. 52(2):187-93.