Recombinant Mouse CD30 Ligand/TNFSF8 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0667

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD30 Ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD30 Ligand protein Gln68-Asp239 (Accession# P32972) with a His tag at the N-terminus.
• GeneID: 21949
• Accession: P32972
• PredictedSize: 20.5 kDa
• SDS-PAGE: 27-42 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily. It is the cytokine that binds to TNFRSF8/CD30. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.

References:

1. Ezogelin Oflazoglu, Iqbal S Grewal, Hanspeter Gerber. (2009) Adv Exp Med Biol. 647:174-85.
2. Nancy D Marín, Luis F García. (2017) Tuberculosis (Edinb). Jan:102:8-15.
3. Jin Duan, Yaxian Gao. (2019) Int Immunopharmacol. Jun:71:350-360.