Recombinant Mouse CD32B/Fcgr2b protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg5259

---

Validation Data Gallery

• 

  Purity of Recombinant Mouse CD32B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD32B protein Thr30-Pro210 (Accession# P08101-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 14130
• Accession: P08101-1
• PredictedSize: 46.6 kDa
• SDS-PAGE: 55-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD32B, also known as FcgammaRIIb or FCGR2B, is a protein that belongs to the family of Fc receptors. It is a type I transmembrane protein and a member of the immunoglobulin superfamily of low-affinity immunoglobulin gamma Fc receptors. This protein acts as a low-affinity receptor for the Fc region of immunoglobulin gamma complexes. It plays a crucial role in immune responses, particularly in the phagocytosis of immune complexes. CD32B is involved in several processes, including Fc-gamma receptor signaling pathway involved in phagocytosis, negative regulation of B cell receptor signaling pathway, and regulation of dendritic spine maintenance. It is expressed in various cell types, such as B cells, myeloid cells, and is also found on the surface of B cell leukemia/lymphoma cells. The protein is encoded by the FCGR2B gene and is implicated in various diseases, including autoimmune thrombocytopenic purpura, glomerulonephritis, lymphoid leukemia, malaria, and systemic lupus erythematosus.

References:

1.Stopforth RJ. et al. (2016) J Clin Immunol. 36 Suppl 1:88-94.
2.Tutt AL. et al. (2015) J Immunol. 195(11):5503-16.
3.Lim SH. et al. (2011) Blood. 118(9):2530-40.