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Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3847

Validation Data Gallery

  • Purity of Recombinant Mouse CD4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CD4 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CD4 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD4 protein Lys27-Thr394 (Accession# P06332-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 12504
Accession P06332-1
PredictedSize 67.5 kDa
SDS-PAGE 70-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

T cell surface glycoprotein CD4, the global membrane glycoprotein, plays an important role in the immune response, performing multiple functions in response against external and internal attacks. CD4+ T cells are the main cells involved in the acquired immune response and play an important role in body development and homeostasis. Upon T cell antigen receptor stimulation and the synergistic activity of cytokines, naive CD4+ T cells differentiate into distinct subsets, including Th1, Th2, Th17, and regulatory T (Treg) cells.

References:

1. Kurup S, et al. Nature reviews Immunology. 2019;19:457–71.
2. J B, et al. Nature reviews Immunology. 2018;18:635–47.