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Recombinant Mouse Calreticulin protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3005

Validation Data Gallery

  • Purity of Recombinant Mouse Calreticulin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Calreticulin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Calreticulin protein Asp18-Leu416(Accession# P14211) with a rabbit IgG Fc tag at the C-terminus.
GeneID 12317
Accession P14211
PredictedSize 72.4 kDa
SDS-PAGE 70-90 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Calreticulin (CRT) is a multifunctional protein with diverse roles in cellular physiology and pathology. It is primarily localized to the endoplasmic reticulum (ER), where it plays a crucial role in protein folding, calcium homeostasis, and the unfolded protein response. CRT also has functions outside the ER, including cell surface expression and extracellular matrix interactions, which are implicated in adhesion, migration, phagocytosis, and immunoregulation. CRT's dysfunction has been linked to various diseases, particularly cancer. It is considered a potential diagnostic marker and therapeutic target for cancer due to its role in cellular proliferation, differentiation, and apoptosis.

References:

1.Nauseef WM. et al. (1995). J Biol Chem. 270(9):4741-4747. 
2.Sun J. et al. (2017). Pharmazie. 72(9):503-510.
3.Gold LI. et al. (2010). FASEB J. 24(3):665-683.