Mouse GM-CSF ELISA Kit
Cat no : KE10119
Synonyms
Csf2,GM-CSF,Colony Stimulating Factor 2,CSF 2,Csfg
Validation Data Gallery
Product Information
KE10119 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GM-CSF. The assay recognizes mouse GM-CSF. An antibody specific for mouse GM-CSF has been pre-coated onto the microwells. The mouse GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse GM-CSF is added to detect the captured mouse GM-CSF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse GM-CSF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12981 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 80% | 76%-84% |
| Cell culture supernatant | 91% | 87%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 197.9 | 10.4 | 5.3 |
| 2 | 20 | 45.6 | 2.0 | 4.3 |
| 3 | 20 | 11.5 | 1.1 | 9.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 211.7 | 15.7 | 7.4 |
| 2 | 24 | 48.6 | 2.9 | 6.1 |
| 3 | 24 | 9.7 | 1.2 | 12.9 |
Background Information
GM-CSF, also known as CSF2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. GM-CSF is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. GM-CSF has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Csf2,GM-CSF,Colony Stimulating Factor 2,CSF 2,Csfg |