Mouse/Rat IGF1 ELISA Kit

Sensitivity

1.3 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse/Rat

Cat no : KE10032

Synonyms

C730016P09Rik, Igf 1, Igf I, Igf1, insulin like growth factor 1



Product Information

KE10032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Mouse/Rat IGF1 ELISA Kit
Tests 1 X 96 well plate
Sample type Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell lysates, Tissue lysates
Assay type Sandwich
Sensitivity 1.3 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum105%95%-119%
Rat serum103%79%-117%
Tissue lysates103%87%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120550.216.02.9
220164.86.74.1
32078.05.16.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124501.338.47.7
224138.514.110.2
32456.06.110.8

Background Information

Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C730016P09Rik, Igf 1, Igf I, Igf1, insulin like growth factor 1

Publications

SpeciesSample TypeTitle
mouseserum

Biol Pharm Bull

Juzentaihoto Suppresses Muscle Atrophy and Decreased Motor Function in SAMP8 Mice.

Authors - Yasuyo Morita