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Recombinant Mouse IL-23 protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 130-520 ng/mL

Cat no : Eg0687

Validation Data Gallery

  • Purity of Recombinant Mouse IL-23 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-23 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Mouse IL-23 (His tag) at 0.5 μg/mL (100 μL/well) can bind Mouse IL-23R (hFc tag) with a linear range of 130-520 ng/mL.

    Immobilized Mouse IL-23 (His tag) at 0.5 μg/mL (100 μL/well) can bind Mouse IL-23R (hFc tag) with a linear range of 130-520 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse IL-23 (His tag) at 0.5 μg/mL (100 μL/well) can bind Mouse IL-23R (hFc tag) with a linear range of 130-520 ng/mL.
Expression HEK293-derived Mouse IL-23 protein Met23-Ser335 (Mouse IL-12B Accession# P43432)+linker+Leu20-Ala196(Mouse IL-23A Accession# Q9EQ14) with His tag at the C-terminus.
GeneID
Accession P43432(IL-12B) & Q9EQ14(IL-23A)
PredictedSize 58.1 kDa
SDS-PAGE 60-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 23 (IL-23) is a member of the IL-12 cytokine family and composed of two subunits, IL-12p40 and IL-23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T cells called Th17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-β and IL-6. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages.

References:

1. Kleinschek MA. et al. (2006). J Immunol. 176:1098-106.
2. Langrish CL. et al. (2005). J Exp Med. 201:233-40.
3. Parham C. et al. (2002). Journal of Immunology. 168 (11):5699-708.
4. Cua DJ. et al. (2003). Nature.421:744-8.