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Recombinant Mouse IL-12 protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 1-5 ng/mL

Cat no : Eg0742

Validation Data Gallery

  • Purity of Recombinant Mouse IL-12 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-12 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL.

    Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Measured by its ability to induce IFN-gamma secretion in human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL.
Expression HEK293-derived Mouse IL-12 protein Met23-Ser335 (Mouse IL-12B Accession# P43432)+linker+Arg23-Ala215 (Mouse IL-12A Accession# P43431) with His tag at the C-terminus.
GeneID 16160(IL-12B) & 16159(IL-12A)
Accession P43432(IL-12B) & P43431(IL-12A)
PredictedSize 59.2 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 12 (IL-12) is a heterodimeric cytokine that is capable of inducing a wide range of immune effects. IL-12 itself is composed of one 35 kDa α-chain subunit (p19 or p35) paired with one 40 kDa β-chain subunit (p40). It is a member of the larger IL-12 family, which also includes 3 other cytokines; IL-23, IL-27, and IL-35. IL-12 is a pro-inflammatory cytokine produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. The IL-12 receptors (IL-12R) are made up of IL-12Rβ1 and IL-12Rβ2. The activation of IL-12R causes tyrosine phosphorylation of primarily JAK2 and TYK2, which can then phosphorylate STAT1, STAT3, STAT4, and STAT5. IL-12 has been shown to have multiple functions, including inducing Th1 cell differentiation, increasing activation and cytotoxic capacities of T and NK cells, and inhibiting or reprogramming immunosuppressive cells. IL-12 also induces the production of large amounts of IFN-γ.

References:

1. Schwarz E and Carson WE 3rd. (2022). J Leukoc Biol. 112(3):557-567.
2. Vignali DA and Kuchroo VK. (2012). Nat Immunol. 13(8):722-8.
3. Yan J. et al. (2018). Cold Spring Harb Perspect Biol. 10(7):a028530.
4. Nguyen KG. et al. (2020). Front Immunol. 11:575597.