"Mouse IgG2a isotype control Antibodies" Comparison
View side-by-side comparison of Mouse IgG2a isotype control antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66360-2-Ig targets Mouse IgG2a isotype control in WB, IHC, FC , IP, ChIP, ELISA applications and shows reactivity with megathura crenulata samples.
| Tested Reactivity | megathura crenulata |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Mouse IgG2a isotype control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Mouse IgG2a isotype control antibody 66360-2-Ig | Download protocol |
| WB protocol for Mouse IgG2a isotype control antibody 66360-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Mouse IgG2a isotype control Monoclonal antibody (11A1B2), catalog number 66360-2-Ig, has 11 total citations. Citations appear in Nature, Nature Cell Biology, Nature Communications, Plant Biotechnology Journal, Pharmacological Research, Biosensors and Bioelectronics, The Plant Journal, Plant Physiology and Biochemistry, Journal of Vascular and Interventional Radiology, Anatomical Record, and International Immunopharmacology. Research fields span molecular biology, cardiovascular disease, plant science, cancer metabolism, biosensing, immunology, and developmental biology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression. | ||
Nat Commun Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire | ||
Pharmacol Res YBX1-LDHB axis orchestrates pyruvate production from lactate to promote ICC initiation and development. | ||
Biosens Bioelectron Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes. |
Reviews
What customers say
One reviewer, Niklas Hartmann, rated this product 5 stars in July 2023. He used it for immunofluorescence and flow cytometry on mouse and human heart tissue at a dilution of 1:200–1:400 (lot 10028300). His comment noted that it provided better staining control compared to using only a secondary antibody control.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Niklas (Verified Customer) (07-31-2023) | better staining control than secondary only control
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