Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

megathura crenulata

Applications

WB, FC , IP, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-Ig

Synonyms

Mouse IgG2a


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  • Western Blot (WB) analysis of various lysates using Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig)

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig)

    FC experiment of human PBMCs using 66360-2-Ig

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig, Clone:11A1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or unstained (blue). Cells were not fixed.

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Tested Applications

Positive WB detected in
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-2-Ig targets Mouse IgG2a isotype control in WB, FC , IP, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Reactivity megathura crenulata
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2a isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Biosens Bioelectron

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Authors - Lei Wang
IP

Nature

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1

Authors - Milan Hluchý

Nat Commun

Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire

Authors - Thomas Eden

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Niklas (Verified Customer) (07-31-2023)

better staining control than secondary only control

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: 1:200-1:400
  • Cell Tissue Type: mouse and human heart