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Recombinant Mouse L1cam protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3022

Validation Data Gallery

  • Purity of Recombinant Mouse L1cam was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse L1cam was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse L1cam protein Ile20-Glu1123 (Accession# P11627) with a rabbit IgG Fc tag at the C-terminus.
GeneID 16728
Accession P11627
PredictedSize 150.1 kDa
SDS-PAGE 140-250 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

L1cam (the L1 cell adhesion molecule), is the prototype member of the L1-family of closely related neural adhesion molecules. L1cam is a 200–220 kDa transmembrane glycoprotein of the immunoglobulin (Ig) superfamily composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail. L1cam plays an essential role in the development of the nervous system but it is also highly relevant for the progression of human tumors. L1cam is not expressed in most normal tissues during homeostasis, including in rapidly proliferating tissues such as the intestinal epithelium.

References:

1. Doberstein K, et al. (2015). Mar 1;136(5):E326-39. 
2. Kiefel H, et al. (2012). Cell Adh Migr. Jul-Aug;6(4):374-384.
3. Ganesh K, et al. (2020). Nat Cancer. 2020 Jan;1(1):28-45.