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Recombinant Mouse Mesothelin protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2864

Validation Data Gallery

  • Purity of Recombinant Mouse Mesothelin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Mesothelin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Mesothelin was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Mesothelin was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Mesothelin protein Asp298-Ser600 (Accession# Q61468) with a rabbit IgG Fc tag at the C-terminus.
GeneID 56047
Accession Q61468
PredictedSize 60.2 kDa
SDS-PAGE 62-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Mesothelin (MSLN) is a cell-surface glycoprotein with normal expression restricted to mesothelial cells lining the pleura, peritoneum, and pericardium. MSLN is highly expressed in mesothelioma, lung, pancreas, breast, ovarian, and other cancers as a cell-surface antigen implicated in tumor invasion. MSLN is considered to be involved in several mechanisms of cancer pathogenesis. MSLN is emerging as an attractive target for cancer immunotherapy, considering its low expression on normal mesothelial cells and high expression in a broad spectrum of solid tumors.

References:

1.Morello A. et al. (2016) Cancer Discov. 6(2):133-146. 
2.Klampatsa A. et al. (2021) Expert Opin Biol Ther. 21(4):473-486.
3.Cao B. et al. (2021) Int J Biol Sci. 17(14):3850-3861.