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Recombinant Mouse tPA protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3025

Validation Data Gallery

  • Purity of Recombinant Mouse tPA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse tPA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse tPA protein Leu18-Gln559 (Accession# P11214) with a rabbit IgG Fc tag at the C-terminus.
GeneID 18791
Accession P11214
PredictedSize 87.2 kDa
SDS-PAGE 27-30 kDa, 35-37 kDa and 58-68 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

tPA (Tissue-type plasminogen activator), also known as Plat, is a serine proteinase found in the intravascular space and the CNS. In the brain tPA is found in the endothelial cell–basement membrane–astrocyte interface, where it modulates cerebrovascular tone and the permeability of the blood-brain barrier. tPA is a key component in the dissolution of blood clots, primarily by catalyzing the conversion of plasminogen to plasmin, the primary enzyme involved in dissolving blood clots.

References:

1. Echeverry R, et al. (2010). J Clin Invest. Jun;120(6):2194-205. 
2. Bugge, T. H., et al. (1996). Proc Natl Acad Sci U S A. Jun 11;93(12):5899-904.
3. Sashindranath M, et al. (2012). Brain. Nov;135(Pt 11):3251-64. 
4. Collen D, et al. (2004). J Thromb Haemost. Apr;2(4):541-6.