Recombinant Mouse Tie-2/CD202b protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1514

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Tie-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Tie-2 protein Ala23-Lys744 (Accession# Q02858) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 21687
• Accession: Q02858
• PredictedSize: 106.9 kDa
• SDS-PAGE: 120-140 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tie-2, also known as CD202b or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. TIE-2 is a tyrosine kinase receptor expressed mainly by endothelial cells, cancer cells, and some immune cells like monocytes. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 is selectively expressed in endothelial cells and gene deficiency of Tie-2 leads to embryonic lethality due to abnormal vascular development. Ang-1, which is secreted from perivascular cells, has been identified as the major ligand for Tie-2. Binding of Ang-1 to Tie-2 elicits different downstream signaling pathways mainly regulated by Akt, which induces pro-angiogenic responses including the promotion of endothelial cell migration, tube formation and survival.

References:

1. Dumont DJ. et al. (1992) Oncogene. 7(8):1471-80.
2. Duran CL. et al. (2021) Cancers (Basel). 13(22):5730.
3. Sato TN. et al. (1995) Nature. 376(6535):70-4.
4. Isidori AM. et al. (2016) J Endocrinol Invest. 39(11):1235-1246.
5. Fukuhara S. et al. (2010) Histol Histopathol. 25(3):387-96.