Mouse Transferrin ELISA Kit
Cat no : KE10207
Synonyms
Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, Tf, Trf
Validation Data Gallery
Product Information
KE10207 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Transferrin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Transferrin. The assay recognizes mouse Transferrin. An antibody specific for mouse Transferrin has been pre-coated onto the microwells. The mouse Transferrin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Transferrin is added to detect the captured mouse Transferrin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Transferrin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.13 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22041 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 103% | 97%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 11.66 | 0.52 | 4.47 |
| 2 | 8 | 2.82 | 0.21 | 7.33 |
| 3 | 8 | 1.35 | 0.12 | 8.74 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.64 | 0.72 | 6.22 |
| 2 | 16 | 2.73 | 0.20 | 7.35 |
| 3 | 16 | 1.40 | 0.13 | 9.45 |
Background Information
Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, Tf, Trf |