Recombinant Mouse TSLP protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3312

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Validation Data Gallery

• 

  Purity of Recombinant Mouse TSLP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse TSLP protein Tyr20-Glu140 (Accession# Q9JIE6) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 53603
• Accession: Q9JIE6
• PredictedSize: 40.2 kDa
• SDS-PAGE: 43-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Thymic stromal lymphopoietin (TSLP) is a 24 kDa monomeric glycoprotein that was originally identified as a product of a thymic stromal cell line. TSLP mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is expressed in a number of tissues including heart, liver and prostate. Human TSLP shares only 38% aa sequence identity with mouse TSLP in the mature region. TSLP has been shown to expand the CD4+ subset of lymphocytes and thymocytes and thus regulate CD4+ homeostasis. Overexpression of TSLP causes imbalances in lymphopoiesis and myelopoiesis.

References:

1. Sims J E. et al. (2000) J Exp Med. 192:671.
2. Reche P A. et al.(2001) J Immunol. 167:336-343.
3. Quentmeier H. et al. (2001) Leukemia. 15:1286-1292.
4. Allakhverdi Z. et al. (2007)J Exp Med. 204:253-258.
5. Quentmeier H. et al. (2001) Leukemia. 15:1286-1292.
6. Reche P A. et al. (2001) J Immunol. 167:336.
7. Al-Shami A. et al. (2004) J Exp Med. 200:159.
8. Osborn M J. et al. (2004) Blood. 103:843.