N6-Methyladenosine (m6A) antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Not Species Specific

Applications

Dot Blot

Cat No : 61495,61496,61995

Synonyms

N6-Methyladenosine, 6-Methyladenosine, 6-meA, 6meA, 6mA, RNA, mRNA, FTO, Fat mass and obesity-associated protein, ALKBH, METTL3, demethylation, N6, antibody, polyclonal, pAb, dot blot, db, sample

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Validation Data Gallery

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  N6-Methyladenosine (m6A) antibody (pAb) tested by RNA dot blot analysis. 3.3ng of a 22 nt RNA containing four repeats of the GAC consensus for N6-methyladenosine within its sequence was spotted onto a membrane and probed with N6-Methyladenosine antibody (1 ug/ml). The sequence contained various adenosine modifications at all four positions as indicated. Lane 1: Adenosine. Lane 2: N6-methyladenosine. Lane 3: 1-methyladenosine. Lane 4: 2’-O-methyladenosine. Lane 5: inosine

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  N6-Methyladenosine (m6A) antibody (pAb) tested by DNA dot blot analysis. Single-stranded DNA oligonucleotides (amount of oligo in pmoles listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with N-6-methyladenosine antibody (1 ug/ml dilution). Lane 1: DNA containing a single 6-methyladenosine. Lane 2: DNA containing unmethylated adenosine. Lane 3: DNA containing a single 1-methyladenosine.

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Product Information

• Tested Applications: Dot Blot

  Applications Validated by Active Motif: DB: 1 - 2 ug/ml dilution For N6-Methyladenosine (m6A), we also offer AbFlex N6-Methyladenosine (m6A) Recombinant Antibody (rAb). For details, see Catalog No. 91261.

• Tested Reactivity: Not Species Specific
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: This antibody was raised against 6-Methyladenosine conjugated to KLH.
• Full Name: N6-Methyladenosine (m6A) antibody (pAb)
• Synonyms: N6-Methyladenosine, 6-Methyladenosine, 6-meA, 6meA, 6mA, RNA, mRNA, FTO, Fat mass and obesity-associated protein, ALKBH, METTL3, demethylation, N6, antibody, polyclonal, pAb, dot blot, db, sample
• Molecular weight: Not Applicable
• GenBank accession number: N/A
• RRID: AB_2793658
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

N6-Methyladenosine (m6A) ) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.